Publications & Grants

Collaborative RRM Member Peer-reviewed Publications

Krawetz RJ, Wu YE, Bertram KL, Shonak A, Masson AO, Ren G, Leonard C, Kapoor M, Matyas JR, Salo PT. Synovial mesenchymal progenitor derived aggrecan regulates cartilage homeostasis and endogenous repair capacity. (2022) Cell Death Dis. 2022 May 18;13(5):470. doi: 10.1038/s41419-022-04919-1.
Rahmani W, Chung H, Sinha S, Bui-Marinos MP, Arora R, Jaffer A, Corcoran, JA, Biernaskie J*, Chun J.*. (2022). Attenuation of SARS-CoV-2 infection by losartan in human kidney organoids. iScience, 25(2):103818. [IF=4.5] https://doi.org/10.1016/j.isci.2022.103818.
Nath SC, Day B, Harper L, Yee J, Hsu CY, Larijani L, Rohani L, Duan N, Kallos MS, Rancourt DE. (2021) Fluid shear stress promotes embryonic stem cell pluripotency via interplay between β-catenin and vinculin in bioreactor culture. Stem Cells 39:1166-1177.
Sakib S, Voigt A, de Lima E Martins Lara N, Su L, Ungrin M, Rancourt D, Dobrinski I. (2021). The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway. Int J Mol Sci. 17:13549. https://doi.org/10.3390/ijms222413549 PubMed PMID: 34948348; PubMed Central PMCID: PMC8708394.
Voigt, A.L., D.A. Kondro, D. Powell, H. Valli-Pulsaki, M. Ungrin, J.-B. Stukenborg, C. Klein, I.A. Lewis, K.E. Orwig & I. Dobrinski. (2021). Unique metabolic phenotype and metabolic maturation in juvenile male germ cells. FASEB J. 35 (5): e21513 https://doi.org/10.1096/fj.202002799R PMCID: PMC8212869; NIHMSID: NIHMS1709299; PMID: 33811704
Shin, W., W. Alpaugh, L.J. Hallihan, S. Sinha, E. Crowther, G.R. Martin, T. Scheidl-Yee, X. Yang, G. Yoon, T. Goldsmith, N.D. Berger, L.G.N. De Almeida, A. Dufour, I. Dobrinski, M. Weinfeld, F.R. Jirik, J. Biernaskie. (2021). PNKP is required for maintaining the integrity of progenitor cell populations in adult mice. Life Science Alliance 4 (9): e202000790 https://doi.org/10.26508/lsa.202000790
Alpaugh, W., A.L. Voigt, R. Dardari, L. Su, I. Al-Khatib, W. Shin, T.M. Goldsmith, K.M. Coyle, L.A. Tang, T.E. Shutt, C. Klein, J. Biernaskie & I. Dobrinski. (2021). Loss of Ubiquitin Carboxy-Terminal Hydrolase L1 impairs Long-Term Differentiation Competence and Metabolic Regulation in Murine Spermatogonial Stem Cells. Cells 10, 2265. https://doi.org/10.3390/cells10092265 PubMed PMID: 34571914; PubMed Central PMCID: PMC8465610.
Colter J, Murari K, Biernaskie J, Kallos MS. (2021) Induced pluripotency in the context of stem cell expansion bioprocess development, optimization, and manufacturing: a roadmap to the clinic. NPJ Regen Medicine 6(1):72. doi: 10.1038/s41536-021-00183-7. [IF=9.3]
Sparks HD, Sigaeva T, Tarraf S, Mandla S, Pope H, Hee O, DiMartino ES, Biernaskie J, Radisic, M, Scott WM. (2021). Biomechanics of wound healing in an equine limb model: effect of location and treatment with a peptide-modified collagen-chitosan hydrogel. ACS Biomaterials Science & Engineering, 7(1):265-278. [I.F.= 4.5]
Borys BS, Dang T, So T, Rohani L, Ravey T, Walsh T, Argiropoulos B, Rancourt DE, Jung S, Hashimura Y, Lee B and Kallos MS. (2020). Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors. Stem Cell Res Ther 12: 55.
Borys BS, So T, Colter J, Dang T, Roberts E, Revey T, Larijani L, Krawetz R, Lewis I, Argiropoulos B. Rancourt DE, Jung S, Hashimura Y, Lee B and Kallos MS. (2020). Optimized Serial Expansion of Human Induced Pluripotent Stem Cells Using Low Density Inoculation to Generate Clinically Relevant Quantities in Vertical-Wheel Bioreactors. Stem Cell Transl Med 9: 1036-1052. doi: 10.1002/sctm.19-0406. Epub 2020 May 22.
Borys BS, So T, Roberts EL, Ferrie L, Larijani L, Abraham B, Krawetz R, Rancourt DE, Kallos MS. (2020). Large-scale expansion of feeder-free mouse embryonic stem cells serially passaged in stirred suspension bioreactors at low inoculation densities directly from cryopreservation. Biotechnol Bioeng. 2020 May;117(5):1316-1328. doi: 10.1002/bit.27279. Epub 2020 Feb 11.
Goldsmith, T.M., S. Sakib, D. Webster, D.F. Carlson, F. Van Der Hoorn & I. Dobrinski. (2020). A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids. Cell & Tiss. Res. 380(1), 191-200. https://doi.org/10.1007/s00441-019-03121-8 PubMed PMID: 31900662; PubMed Central PMCID: PMC7815324.
Hagner, A., W. Shin, S. Sinha, W. Alpaugh, M. Workentine, S. Abbasi, W. Rahmani, N. Agbalyahn, N. Sharma, H. Sparks, J. Yoon, E. Labit, J. Cobb, I. Dobrinski & J. Biernaskie. (2020). Transcriptional profiling of the adult hair follicle mesenchyme reveals R-spondin as a novel regulator of dermal progenitor function. iScience 23, 101019. https://doi.org/10.1016/j.isci.2020.101019 PubMed PMID: 32289736; PubMed Central PMCID: PMC7155209.
Uchida, A., S. Sakib, E. Labit, S.Abbasi, W. Scott, M. Underhill, J. Biernaskie & I. Dobrinski. (2020). Development and Function of Smooth Muscle Cells is Modulated by Hic1 in Mouse Testis. Development 147, dev185884. https://doi.org/10.1242/dev.185884 PubMed PMID: 32554530; PubMed Central PMCID: PMC7375483.
Borys BS, Le A, Roberts EL, Rohani L Hsu C, Wyma, AA, Rancourt DE, Gates, ID, Kallos MS. (2019). Using computational fluid dynamic (CFD) modeling to understand murine embryonic stem cell aggregate size and pluripotency distributions in stirred suspension bioreactors. J Biotechnol 304: 16-27.
Gao L, Nath SC, Jiao X, Zhou R, Nishikawa S, Krawetz R, Li X, Rancourt DE. (2019). Post-Passage ROCK Inhibition Induces Cytoskeletal Aberrations and Apoptosis in Human Embryonic Stem Cells. Stem Cell Research 41: 101641. doi: 10.1016/j.scr.2019.101641. Epub 2019 Oct 28.
Rohani L, Borys BS, Razian G, Naghsh P, Mahranju P, Johnson AA, Holland H, Liu S, Li J, Kallos MS, Rancourt DE. (2019) Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells. Nat Commun Biol
Lee PS, Hess R, Friedrichs J, Haenchen V, Eckert H, Cuniberti G, Rancourt D, Krawetz R, Hintze V, Gelinsky M, Scharnweber D. (2019). Recapitulating bone development events in a customised bioreactor through interplay of oxygen tension, medium pH, and systematic differentiation approaches. J Tissue Eng Regen Med. 2019 Sep;13(9):1672-1684. doi: 10.1002/term.2921. Epub 2019 Jul 15.
Sakib, S., A. Uchida, P. Valenzuela-Leon, Y. Yu, H. Valli-Pulaski, K. Orwig, M. Ungrin & I. Dobrinski. (2019). Formation of organotypic testicular organoids in microwell culture. Biol. Reprod. 100(6): 1648-16160. DOI: 10.1093/biolre/ioz053 PubMed PMID: 30927418; PubMed Central PMCID: PMC7302515.
Burrell, K., R. Dardari, T. Goldsmith, D. Toms, D.A.F. Villagomez, W.A. King, M. Ungrin, F.D. West, & I. Dobrinski. (2019). Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells. Stem Cells & Dev. 28 (18): 1264-1275. DOI: 10.1089/scd.2019.0111. PMID:31264514; PMCID: PMC6751395
Agabalyan NA*, Sparks H*, Tarraf S, Rosin NL, Anker K, Yoon G, Burnett LN, Nickerson D, Di Martino ES, Gabriel VA, Biernaskie J. (2019). Adult human dermal progenitor cell transplantation modulates the functional outcome of split thickness skin xenografts. Stem Cell Reports. 13, 1068-1082. [I.F.= 5.5]
Sparks JD*, Fraz Anjum*, Vallmajo-Martin Q, Ehrbar M, Abbasi S, Kallos MS*, Biernaskie J*. (2019) Flowable polyethylene glycol hydrogels support the in vitro survival and proliferation of dermal progenitor cells in a mechanically-dependent manner. ACS Biomaterials Science & Engineering, 5 (2) 950-958. [IF=4.4]
Sinha S, Nuñez Martinez CM, Hartley RL, Quintana Alvarez RJ, Yoon G, Biernaskie JA, Nickerson D, Gabriel VA. (2019). Epidemiological analysis of pediatric burns in the Dominican Republic reveals a demographic profile at significant risk for electrical burns. Burns. 2019 Mar;45(2):471-478. doi: 10.1016/j.burns.2018.03.014.
Stratton JA*, Sinha S*, Shin W, Labit E, Chu T-H, Shah PT, Midha R, Biernaskie J. (2019). Droplet barcoding-based single cell transcriptomics of adult mammalian tissues. Journal of Visual Experimentation,143. doi: 10.3791/58709.
Hsu C, Walsh T, Borys B, Kallos M, Rancourt DE. (2018). An integrated approach towards the bio manufacturing of engineered cell therapy products in a continuous stirred suspension bioreactor. Mol Ther - Meth Clin Dev 9: 376-389.
Bertram KL, Narendran N, Tailor P, Jablonski C, Leonard C, Irvine E, Hess R, Masson AO, Abubacker S, Rinker K, Biernaskie J, Yates RM, Salo P, Narendran A, Krawetz RJ. (2018). 17-DMAG regulates p21 expression to induce chondrogenesis in vitro and in vivo. Dis Model Mech. 2018 Oct 8;11(10):dmm033662. doi: 10.1242/dmm.033662.
Jorgenson KD, Hart DA, Krawetz R, Sen A. (2018). Production of Adult Human Synovial Fluid-Derived Mesenchymal Stem Cells in Stirred-Suspension Culture. Stem Cells Int. 2018 Mar 27;2018:8431053. doi: 10.1155/2018/8431053. eCollection 2018.
Stratton JA, Assinck P, Sinha S, Kumar R, Moulson A, Patrick N, Raharjo E, Chan J, Midha R, Tetzlaff W, Biernaskie J. (2018) Factors within the endoneurial microenvironment act to suppress tumorigenesis of MPNST. Frontiers in Cellular Neuroscience, 12:356. doi: 10.3389/fncel.2018.00356. [IF=4.5]
Stratton, JA, Holmes A, Rosin NL, Sinha S, Vohra M, Burma NE, Trang T, Midha R, Biernaskie J. (2018) Macrophages regulate Schwann cell maturation following nerve injury. Cell Reports 24(10):2561-2572.e6. [IF=8.1]
Lee PS, Eckert H, Hess R, Gelinsky M, Rancourt D, Krawetz R, Cuniberti G, Scharnweber D. (2017). Developing a Customized Perfusion Bioreactor Prototype with Controlled Positional Variability in Oxygen Partial Pressure for Bone and Cartilage Tissue Engineering. Tissue Eng Part C Methods. 2017 May;23(5):286-297. doi: 10.1089/ten.TEC.2016.0244.
O'Brien K, Tailor P, Leonard C, DiFrancesco LM, Hart DA, Matyas JR, Frank CB, Krawetz RJ. (2017). Enumeration and Localization of Mesenchymal Progenitor Cells and Macrophages in Synovium from Normal Individuals and Patients with Pre-Osteoarthritis or Clinically Diagnosed Osteoarthritis. Int J Mol Sci. 2017 Apr 5;18(4):774. doi: 10.3390/ijms18040774.

Book chapters

Image Analysis Method for Evaluating Heterogeneous Growth and Differentiation of Embryonic Stem Cell Cultures. Hunt, MM., Meng, G., Rancourt, DE., Gates, ID., Kallos, MS. Best of BIOT award, American Chemical Society, Division of Biochemical Technology, eBook Chapter 10 in Developments in Biotechnology and Bioprocessing. 2013, American Chemical Society. p. 165-181. Chapter DOI: 10.1021/bk-2013-1125.ch010, ACS Symposium Series, Vol. 1125, ISBN13: 9780841229105 eISBN: 9780841229112, Publication Date (Web): March 8, 2013
Bioreactor Expansion of Skin Derived Precursor Schwann Cells. Walsh, T, Biernaskie, J, Midha, R, Kallos, MS., Methods in Molecular Biology (2016), Human Press, DOI: 10.1007/7651_2016_355

Grants

2022-2024: Developmental origins of vertebral deformities – defining the critical role of the NOTCH signaling pathway. ACHRI Grand Challenge Catalyst – Seedling award. (2 Years; $50,000). PI: Jack Chu. Co-PIs: Alex Lohman, Mark Ungrin.
2021-2023: Establishing a cervid stem cell-derived infection model for chronic wasting disease prions. New Frontiers in Research Fund – Exploration. (2 Years; $200,000). PI: Sabine Gilch. Co-PI: Jack Chu. Co-Applicant: Rob McCorkell.
2021-2023: Developmental origins of congenital heart disease - defining the critical role of the placenta. ACHRI Grand Challenge Catalyst – Branch award. (2 Years; $200,000). PI: Myriam Hemberger. Co-PIs: Wendy Dean, Antoine Dufour, Mike Kallos, Mark Ungrin, Jack Chu.
2021: Centre for Cell Therapy Translation (C2T2). Canadian Foundation for Innovation – Infrastructure fund (5 years; $4,579,508). PIs: Jeff Biernaskie, Mike Kallos. Co-Applicants: Roman Krawetz, Ina Dobrinski, Derrick Rancourt, Vince Gabriel, Paul Fedak, Holly Sparks, Mark Ungrin.
2021: Role for Lipid Droplet Associated IL-32 in Diabetic Kidney Disease. Canadian Institute of Health Research (CIHR) Project Grant ($715,275). PI: Justin Chun. Collaborator: Jeff Biernaskie.
2020-2025: Transplantation of Testis Stem Cells in Large Animals. NIH/ORIP. ($ 1,024,525). PI: Ina Dobrinski. Collaborators: Mark Ungrin, Claudia Klein.
2018-2023: Understanding dermal progenitor biology towards improved skin wound healing. CIHR Project Grant (5 Years, $749,700). PI: Jeff Biernaskie. Co-Applicant: Vince Gabriel.
2018-2023: Understanding Schwann cell heterogeneity for improved nerve repair. CIHR Project Grant (5 Years; $1,077,120). PI: Rajiv Midha. Co-Investigator: Jeff Biernaskie.
2017-2022: A system for culturing mammalian spermatogonial cells. NIH/NICHD ($ 702,880). PI: Ina Dobrinski. Collaborators: Mark Ungrin, Derrick Rancourt, Mike Kallos.
2016-2019: Derivation and Culture of Naïve Pluripotent Stem Cells in Stirred Suspension Bioreactors. CIHR ($ 351,690). PI: Derrick Rancourt. Co-PI: Mike Kallos.
2016-2019: Derivation and Culture of Naïve Pluripotent Stem Cells in Stirred Suspension Bioreactors. Natural Science and Engineering Research Council. ($ 351,689). PI: Derrick Rancourt. Co-PI: Mike Kallos.
A bioprocess that optimizes safe Human iPSCs for the treatment of osteoporotic fractures. CIHR/IMHA. ($100,000). PI: Roman Krawetz. Co-PI: Mike Kallos.
A bioprocess that optimizes safe Human iPSCs for the treatment of osteoporotic fractures. CIHR. ($695,000). PI: Roman Krawetz. Co-PI: Mike Kallos.